PPIF Knockout cell line(Caco-2)

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PPIF Knockout cell line(Caco-2) Order

PPIF Knockout cell line(Caco-2)
Catalog#
YKO-H342
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
PPIF Knockout cell line(Caco-2)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
PPIF Knockout cell line (Caco-2)
Cell Morphology
Epithelial-like, adherent
Passage ratio
1:2~1:4
Species
Human
Gene Name
PPIF
Gene ID
10105
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
77%MEM+20%FBS+1%Glutamax+1%Sodium Pyruvate+1%NEAA
Accession
CVCL_D8FC(Cellosaurus)
ExPASy Cellosaurus Database
Is a valuable online resource that compiles detailed information on over 100,000 cell lines from reputable cell banks such as ATCC, ECACC, and DSMZ. The database provides information including descriptions, origins, and characteristics of the cell lines, with broad reference value and scientific reliability. Additionally, the database provides information on cell line authentication, STR profiles, and potential contamination risks, helping researchers ensure the trustworthiness of the cell lines used in their experiments.
Target region
Knockout region: Exon 1 (based on transcript PPIF-201)
Validation: PCR+Sanger Sequencing
PPIF Knockout cell line(Caco-2) Target region
STR Profile
Click to download the STR appraisal report
Loci STR Info (Sample Cell)
Sample Cell Line:Caco-2		 STR Info (Cell bank)
Cell Line:CACO-2
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 12131213
D13S317 1114111413.0
D7S820 11121112
D16S539 12121213
VWA 16181618
TH01 6666
AMEL XXXX
TPOX 911911
CSF1PO 11111111
D12S391 1723
FGA 1919
D2S1338 1725
D21S11 3030
D18S51 1212
D8S1179 1212
D3S1358 1717
D6S1043 1212
PENTAE 77
D19S433 1515
PENTAD 99
D1S1656 1516
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the PPIF gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Caco-2 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, PPIF gene knockout PPIF Knockout cell line (Caco-2) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
PPIF Knockout cell line(Caco-2)
PPIF Knockout cell line(Caco-2)
Catalog#
YKO-H342
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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